Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

Xiaomin Xu; Andrada But; Ron Wever; Frank Hollmann

doi:10.1002/cctc.201902194

Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

Xiaomin Xu, Andrada But, Ron Wever, Frank Hollmann^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and k_cat of 75 s⁻¹ were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.

Original language	English
Pages (from-to)	2180-2183
Journal	ChemCatChem
Volume	12
Issue number	8
DOIs	https://doi.org/10.1002/cctc.201902194
Publication status	Published - 2020

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Keywords

biocatalysis
glutamic acid
nitriles
oxidative decarboxylation
vanadium chloroperoxidase

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10.1002/cctc.201902194

cctc.201902194taverneFinal published version, 1.44 MB

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title = "Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid",

abstract = "The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and kcat of 75 s−1 were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.",

keywords = "biocatalysis, glutamic acid, nitriles, oxidative decarboxylation, vanadium chloroperoxidase",

author = "Xiaomin Xu and Andrada But and Ron Wever and Frank Hollmann",

note = "Green Open Access added to TU Delft Institutional Repository {\textquoteleft}You share, we take care!{\textquoteright} – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.",

year = "2020",

doi = "10.1002/cctc.201902194",

language = "English",

volume = "12",

pages = "2180--2183",

journal = "ChemCatChem",

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T1 - Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

AU - Xu, Xiaomin

AU - But, Andrada

AU - Wever, Ron

AU - Hollmann, Frank

N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

PY - 2020

Y1 - 2020

N2 - The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and kcat of 75 s−1 were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.

AB - The chemoenzymatic oxidative decarboxylation of glutamic acid to the corresponding nitrile using the vanadium chloroperoxidase from Curvularia inaequalis (CiVCPO) as HOBr generation catalysts has been investigated. Product inhibition was identified as major limitation. Nevertheless, 1630000 turnovers and kcat of 75 s−1 were achieved using 100 mM glutamate. The semi-preparative enzymatic oxidative decarboxylation of glutamate was also demonstrated.

KW - biocatalysis

KW - glutamic acid

KW - nitriles

KW - oxidative decarboxylation

KW - vanadium chloroperoxidase

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DO - 10.1002/cctc.201902194

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JO - ChemCatChem

JF - ChemCatChem

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ER -

Towards Preparative Chemoenzymatic Oxidative Decarboxylation of Glutamic Acid

Abstract

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Keywords

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Expanding the scope of H2O2-driven biocatalysis

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