Towards rational design and scale up of aerobic industrial fermentations: P.chrysogenum as model organism

Amit Deshmukh, W van Winden, Henk Noorman, Cees Haringa, Rob Mudde, Sef Heijnen, Walter van Gulik, Wenjun Tang, J. Xia, J Chu, M Reuss

Research output: Contribution to conferenceAbstractScientific


The objective of this research study is to be able to scale-up and optimize aerobic fermentation processes via computer simulations. As an example the production of penicillin by P. chrysogenum is studied. In vivo kinetics of the cell can be understood by conducting stimulus response and scale down experiments. These experiments provide dynamic information that can be used to estimate parameters of enzyme kinetic models. The transport phenomena inside the bioreactor can by understood by solving hydrodynamic model using computational fluid dynamics (CFD). Thus, with information from intracellular and extracellular environments, an integrated model is solved using CFD. In these simulations, the multiphase flow and transport phenomena taking place inside the bioreactor are simulated, and integrated with the microbial reaction kinetics. The micro-organisms inside the reactor are followed along their trajectories, where they experience a changing environment. The dynamic response of the cells to this environment dictates the productivity and selectivity of the cells. By simulating both the flow in the reactor and the response of the cells, the entire process inside the bioreactor can be assessed and scaled-up and optimization can be done without the need of costly experiments, both in time and financial resources.
Original languageEnglish
Number of pages1
Publication statusPublished - 28 Jul 2016
Event2016 SIMB Annual Meeting and Exhibition - Grand Chenier, 5th Fl (Sheraton New Orleans), New Orleans, United States
Duration: 24 Jul 201628 Jul 2016


Conference2016 SIMB Annual Meeting and Exhibition
Country/TerritoryUnited States
CityNew Orleans
Internet address


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