TY - JOUR
T1 - Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production
AU - Mans, Robert
AU - Daran, Jean Marc G.
AU - Pronk, Jack T.
PY - 2018
Y1 - 2018
N2 - Evolutionary engineering, which uses laboratory evolution to select for industrially relevant traits, is a popular strategy in the development of high-performing yeast strains for industrial production of fuels and chemicals. By integrating whole-genome sequencing, bioinformatics, classical genetics and genome-editing techniques, evolutionary engineering has also become a powerful approach for identification and reverse engineering of molecular mechanisms that underlie industrially relevant traits. New techniques enable acceleration of in vivo mutation rates, both across yeast genomes and at specific loci. Recent studies indicate that phenotypic trade-offs, which are often observed after evolution under constant conditions, can be mitigated by using dynamic cultivation regimes. Advances in research on synthetic regulatory circuits offer exciting possibilities to extend the applicability of evolutionary engineering to products of yeasts whose synthesis requires a net input of cellular energy.
AB - Evolutionary engineering, which uses laboratory evolution to select for industrially relevant traits, is a popular strategy in the development of high-performing yeast strains for industrial production of fuels and chemicals. By integrating whole-genome sequencing, bioinformatics, classical genetics and genome-editing techniques, evolutionary engineering has also become a powerful approach for identification and reverse engineering of molecular mechanisms that underlie industrially relevant traits. New techniques enable acceleration of in vivo mutation rates, both across yeast genomes and at specific loci. Recent studies indicate that phenotypic trade-offs, which are often observed after evolution under constant conditions, can be mitigated by using dynamic cultivation regimes. Advances in research on synthetic regulatory circuits offer exciting possibilities to extend the applicability of evolutionary engineering to products of yeasts whose synthesis requires a net input of cellular energy.
UR - http://resolver.tudelft.nl/uuid:19b09f8e-efce-440d-937b-f10aa442f50d
UR - http://www.scopus.com/inward/record.url?scp=85034452883&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2017.10.011
DO - 10.1016/j.copbio.2017.10.011
M3 - Review article
AN - SCOPUS:85034452883
SN - 0958-1669
VL - 50
SP - 47
EP - 56
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -