TY - JOUR
T1 - Untangling reaction pathways through modern approaches to high-throughput single-molecule force-spectroscopy experiments
AU - Dulin, D
AU - Berghuis, BA
AU - Depken, SM
AU - Dekker, NH
PY - 2015
Y1 - 2015
N2 - Single-molecule experiments provide a unique means for real-time observation of the activity of individual biomolecular machines. Through such techniques, insights into the mechanics of for example, polymerases, helicases, and packaging motors have been gleaned. Here we describe the recent advances in single-molecule force spectroscopy instrumentation that have facilitated high-throughput acquisition at high spatiotemporal resolution. The large datasets attained by such methods can capture rare but important events, and contain information regarding stochastic behaviors covering many orders of magnitude in time. We further discuss analysis of such data sets, and with a special focus on the pause states described in the general literature on RNA polymerase pausing we compare and contrast the signatures of different reaction pathways.
AB - Single-molecule experiments provide a unique means for real-time observation of the activity of individual biomolecular machines. Through such techniques, insights into the mechanics of for example, polymerases, helicases, and packaging motors have been gleaned. Here we describe the recent advances in single-molecule force spectroscopy instrumentation that have facilitated high-throughput acquisition at high spatiotemporal resolution. The large datasets attained by such methods can capture rare but important events, and contain information regarding stochastic behaviors covering many orders of magnitude in time. We further discuss analysis of such data sets, and with a special focus on the pause states described in the general literature on RNA polymerase pausing we compare and contrast the signatures of different reaction pathways.
UR - http://resolver.tudelft.nl/uuid:aecce8f2-e3a9-493e-b295-467bae75633d
U2 - 10.1016/j.sbi.2015.08.007
DO - 10.1016/j.sbi.2015.08.007
M3 - Article
SN - 0959-440X
VL - 34
SP - 116
EP - 122
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -