Use of chemostat data for modelling extracellular-inulinase production by Kluyveromyces marxianus in a high-cell-density fed-batch process

Marco C.M. Hensing, Johannes S. Vrouwenvelder, Chris Hellinga, Johannes P. Van Dijken, Jack T. Pronk

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

Production of extracellular inulinase by low-cell-density (2 kg dry weight·m-3) sucrose-limited chemostat cultures of Kluyveromyces marxianus obeyed saturated kinetics at dilution rates ranging from 0.02 to 0.5 h-1. A non-structured Monod-type equation, describing the relation between specific growth rate and specific extracellular-inulinase production rate, was used to fit experimental data. THis equation was subsequently incorporated in a model for the production of biomass and extracellular inulinase in a high-cell-density (> 100 kg dry weight·m-3) fed-batchculture of K. marxianus grown on sucrose. The model adequately described biomass production in the fed-batch culture. However, the production of extracellular inulinase in the fed-batch process was slightly higher than predicted by the model. This observation may be related to differences in growth conditions between in the chemostat and fed-batch cultures.

Original languageEnglish
Pages (from-to)54-58
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number1
DOIs
Publication statusPublished - 1995

Keywords

  • chemostat
  • extracellular inulinase
  • fed-batch process
  • Kluyveromyces marxianus
  • modelling

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