Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy

Paolo Pozzi, Dean Wilding, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

1 Citation (Scopus)
53 Downloads (Pure)

Abstract

In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance CMOS camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.

Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationEmerging Digital Micromirror Device Based Systems and Applications X
EditorsMichael R. Douglass, Benjamin L. Lee
Place of PublicationBellingham, WA, USA
PublisherSPIE
Number of pages5
Volume10546
ISBN (Electronic)978-1-510615779
DOIs
Publication statusPublished - 2018
EventEmerging Digital Micromirror Device Based Systems and Applications X - San Francisco, United States
Duration: 29 Jan 201830 Jan 2018

Publication series

NameProceedings of SPIE
Volume10546
ISSN (Electronic)1605-7422

Conference

ConferenceEmerging Digital Micromirror Device Based Systems and Applications X
CountryUnited States
CitySan Francisco
Period29/01/1830/01/18

Keywords

  • Adaptive optics
  • Confocal microscopy
  • Digital micromirror devices

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  • Cite this

    Pozzi, P., Wilding, D., Soloviev, O., Vdovin, G., & Verhaegen, M. (2018). Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy. In M. R. Douglass, & B. L. Lee (Eds.), Proceedings of SPIE: Emerging Digital Micromirror Device Based Systems and Applications X (Vol. 10546). [105460D] (Proceedings of SPIE; Vol. 10546). SPIE. https://doi.org/10.1117/12.2288436