Abstract: As a novel volumetric particle image velocimetry technique, single-camera light-field PIV (LF-PIV) is able to acquire three-dimensional flow fields through a single camera. Compared with other multi-camera 3D PIV techniques, LF-PIV has distinct advantages, including concise hardware setup and low optical access requirements. Its capability has proven effective in many experimental investigations. In this study, the use of LF-PIV in measuring a self-similar adverse pressure-gradient turbulent boundary layer (APG-TBL) is demonstrated. Experiments are performed in a large water tunnel at the Laboratory for Turbulence Research in Aerospace and Combustion (LTRAC), Monash University. Sets of 250 light-field PIV image pairs are captured covering both the inner and outer regions of the boundary layer. Instantaneous 3D velocity fields are reconstructed using a GPU accelerated density ray tracing multiplicative reconstruction technique (DRT-MART) and three-dimensional cross-correlation methods. The LF-PIV results are compared with two-dimensional PIV (2D-PIV) measurements of the same flow. Comparable accuracy to 2D-PIV is achieved for first- and second-order velocity statistics above approximately y/ δ1= 1. Graphic abstract: [Figure not available: see fulltext.].