What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments

Bojk Berghuis, Vlad-Stefan Raducanu, Mohamed M. Elshenawy, Slobodan Jergic, Martin Depken, NE Dixon, Samir M. Hamdan, Nynke Dekker

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)
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Synchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. In Escherichia coli, a “replication fork trap” – found within a chromosomal region where forks are allowed to enter but not leave – is set by the protein–DNA roadblock Tus–Ter. The exact sequence of events by which Tus–Ter blocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein–protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus–Ter interactions at the nonpermissive face – interactions that result in a highly stable “locked” complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus–Ter mechanism – leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA–protein system further.
Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalCritical Reviews in Biochemistry and Molecular Biology
Publication statusPublished - 6 Nov 2017


  • DNA–protein interactions
  • DNA replication
  • prokaryotic replication
  • replication termination
  • replisome
  • Tus–Ter
  • single-molecule techniques
  • magnetic tweezers


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