TY - JOUR
T1 - Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa
AU - Costa, Ana Rita
AU - van den Berg, Daan F.
AU - Esser, Jelger Q.
AU - Muralidharan, Aswin
AU - van den Bossche, Halewijn
AU - Bonilla, Boris Estrada
AU - van der Steen, Baltus A.
AU - Haas, Pieter Jan
AU - Brouns, Stan J.J.
AU - More Authors, null
N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
PY - 2024
Y1 - 2024
N2 - Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude of defense systems on phage resistance remains unclear, especially in a clinical context. Using a collection of antibiotic-resistant clinical strains of Pseudomonas aeruginosa and a broad panel of phages, we demonstrate that defense systems contribute substantially to defining phage host range and that overall phage resistance scales with the number of defense systems in the bacterial genome. We show that many individual defense systems target specific phage genera and that defense systems with complementary phage specificities co-occur in P. aeruginosa genomes likely to provide benefits in phage-diverse environments. Overall, we show that phage-resistant phenotypes of P. aeruginosa with at least 19 phage defense systems exist in the populations of clinical, antibiotic-resistant P. aeruginosa strains.
AB - Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude of defense systems on phage resistance remains unclear, especially in a clinical context. Using a collection of antibiotic-resistant clinical strains of Pseudomonas aeruginosa and a broad panel of phages, we demonstrate that defense systems contribute substantially to defining phage host range and that overall phage resistance scales with the number of defense systems in the bacterial genome. We show that many individual defense systems target specific phage genera and that defense systems with complementary phage specificities co-occur in P. aeruginosa genomes likely to provide benefits in phage-diverse environments. Overall, we show that phage-resistant phenotypes of P. aeruginosa with at least 19 phage defense systems exist in the populations of clinical, antibiotic-resistant P. aeruginosa strains.
UR - http://www.scopus.com/inward/record.url?scp=85185859622&partnerID=8YFLogxK
U2 - 10.1126/sciadv.adj0341
DO - 10.1126/sciadv.adj0341
M3 - Article
C2 - 38394193
AN - SCOPUS:85185859622
SN - 2375-2548
VL - 10
SP - eadj0341
JO - Science Advances
JF - Science Advances
IS - 8
M1 - eadj0341
ER -