Biocatalytically induced surface modification of the tobacco mosaic virus and the bacteriophage M13

Valentina Vignali, Barbara S. Miranda, Irene Lodoso-Torrecilla, Cathelijn A.J. Van Nisselroy, Bas Jan Hoogenberg, Sybren Dantuma, Frank Hollmann, Jan Willem De Vries

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Abstract

Engineered viruses are finding an increasing number of applications in basic, translational research and materials science. Genetic and chemical engineering of capsids represents a key point for tailoring the properties of viral particles, but the synthetic efforts and limits accompanying these processes still hinder their usability. Here, a single-step highly selective biocatalytic functionalization approach is described, providing a general platform for virus-acrylate hybrid particles. The tobacco mosaic virus (TMV) and the bacteriophage M13 have been successfully modified via laccase induced free radical formation on the tyrosine residues through single electron oxidation as the initiating step and the free radicals subsequently react with acrylate-based monomers. This new approach can be extended to other biomolecular assemblies with surface exposed tyrosine residues, when the introduction of new functionalities is desired.

Original languageEnglish
Pages (from-to)51-54
JournalChemical Communications
Volume55
Issue number1
DOIs
Publication statusPublished - 2019

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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