Biomolecule electrotransfer to mammalian cells

Research output: ThesisDissertation (TU Delft)

69 Downloads (Pure)


Delivery of biomolecules using pulsed electric fields or electrotransfer has applications such as biomedical engineering, bioprocess engineering and genomic engineering. When a cell is placed in an electric field, the induced transmembrane voltage catalyzes the formation of pores on the cell membrane. This enables the delivery of otherwise cell membrane-impermeable molecules to the cells. Despite the broad significance, a complete biophysical understanding of electrotransfer at a subcellular level and a translation of electroporation as a high-throughput and high-efficiency technique is still lacking. In this dissertation: (i) we unravel how actin networks regulate the cell membrane electropermeability, (ii) we reveal the intracellular biophysical transport mechanisms of electrotransferred DNA cargo, (iii) we present a localized electroporation device where cells are trapped in regions of high electric fields by the flow.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Delft University of Technology
  • Kreutzer, M.T., Supervisor
  • Boukany, P., Supervisor
Thesis sponsors
Award date26 Oct 2022
Print ISBNs978-90-8593-531-5
Publication statusPublished - 2022


  • Electroporation
  • electrotransfer
  • cytoskeleton
  • anomalous diffusion
  • DNA transport
  • microfluidics
  • localized electroporation
  • gene delivery


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