@article{123505ce857745f08b08dae626231525,
title = "Detection of phosphorylation post-translational modifications along single peptides with nanopores",
abstract = "Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.",
author = "Nova, {Ian C.} and Justas Ritmejeris and Henry Brinkerhoff and Koenig, {Theo J.R.} and Gundlach, {Jens H.} and Cees Dekker",
note = "Green Open Access added to TU Delft Institutional Repository {\textquoteleft}You share, we take care!{\textquoteright} – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.",
year = "2023",
doi = "10.1038/s41587-023-01839-z",
language = "English",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature",
}
