Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus

Oleg G. Voronin; Daan J. Van Haaster; Elena E. Karyakina; Wilfred R. Hagen; Arkady A. Karyakin

doi:10.1002/elan.200703967

Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus

Oleg G. Voronin^*, Daan J. Van Haaster, Elena E. Karyakina, Wilfred R. Hagen, Arkady A. Karyakin

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

The direct bioelectrocatalysis by an NAD(P)-reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis [1-4], which were in fact unsuccessful, in our report an effective electrocatalysis by Pyrococcus furiosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm^-2 at 100 mV overpotential and at 75°C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)-independent hydrogenases.

Original language	English
Pages (from-to)	2264-2266
Number of pages	3
Journal	Electroanalysis
Volume	19
Issue number	21
DOIs	https://doi.org/10.1002/elan.200703967
Publication status	Published - Nov 2007

Keywords

Bioelectrocatalysis
Enzyme electrode
Hydrogen equilibrium potential
Hydrogenase
NAD(P)

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title = "Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus",

abstract = "The direct bioelectrocatalysis by an NAD(P)-reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis [1-4], which were in fact unsuccessful, in our report an effective electrocatalysis by Pyrococcus furiosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm-2 at 100 mV overpotential and at 75°C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)-independent hydrogenases.",

keywords = "Bioelectrocatalysis, Enzyme electrode, Hydrogen equilibrium potential, Hydrogenase, NAD(P)",

author = "Voronin, {Oleg G.} and {Van Haaster}, {Daan J.} and Karyakina, {Elena E.} and Hagen, {Wilfred R.} and Karyakin, {Arkady A.}",

year = "2007",

month = nov,

doi = "10.1002/elan.200703967",

language = "English",

volume = "19",

pages = "2264--2266",

journal = "Electroanalysis",

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T1 - Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus

AU - Voronin, Oleg G.

AU - Van Haaster, Daan J.

AU - Karyakina, Elena E.

AU - Hagen, Wilfred R.

AU - Karyakin, Arkady A.

PY - 2007/11

Y1 - 2007/11

N2 - The direct bioelectrocatalysis by an NAD(P)-reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis [1-4], which were in fact unsuccessful, in our report an effective electrocatalysis by Pyrococcus furiosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm-2 at 100 mV overpotential and at 75°C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)-independent hydrogenases.

AB - The direct bioelectrocatalysis by an NAD(P)-reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis [1-4], which were in fact unsuccessful, in our report an effective electrocatalysis by Pyrococcus furiosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm-2 at 100 mV overpotential and at 75°C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)-independent hydrogenases.

KW - Bioelectrocatalysis

KW - Enzyme electrode

KW - Hydrogen equilibrium potential

KW - Hydrogenase

KW - NAD(P)

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U2 - 10.1002/elan.200703967

DO - 10.1002/elan.200703967

M3 - Article

AN - SCOPUS:36148977216

SN - 1040-0397

VL - 19

SP - 2264

EP - 2266

JO - Electroanalysis

JF - Electroanalysis

IS - 21

ER -

Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus

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Keywords

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