TY - JOUR
T1 - Effect of hyaluronic acid on the struvite crystallization
T2 - A structural, morphological, and thermal analysis study
AU - Polat, Sevgi
AU - Eral, Huseyin Burak
PY - 2022
Y1 - 2022
N2 - The struvite crystals constitute one of the common types of urinary stones. Such stones are also referred as “infection stones” due to their tendency to cause infections in urinary tract. A considerable effort has been placed to identify natural or synthetic crystal-growth modifiers for this kind of urinary stone in literature, yet macromolecules commonly found in urine have been underexplored. In the present study, we experimentally focus on how hyaluronic acid, a protein commonly found in urine, alters the struvite crystallization in aqueous solution and in an artificial urine media. By gradually adding ammonium dihydrogen phosphate to a solution containing magnesium chloride hexahydrate, reactive crystallization is carried out in a well-mixed and thermostated vessel at 37 °C. The resulting struvite crystals are characterized structurally by XRD and FTIR as well as morphologically and in terms of their surface charge. In addition, the thermal decomposition behavior of the struvite with and without hyaluronic acid and released volatile products were simultaneously investigated using a TGA/FTIR system. The average activation energy calculated using the Friedman method was 49.2 ± 5.1 kJ/mol. The results of the kinetic and thermodynamic analyses showed that decomposition of the struvite crystals was endothermic and followed the multiple stage reaction mechanism.
AB - The struvite crystals constitute one of the common types of urinary stones. Such stones are also referred as “infection stones” due to their tendency to cause infections in urinary tract. A considerable effort has been placed to identify natural or synthetic crystal-growth modifiers for this kind of urinary stone in literature, yet macromolecules commonly found in urine have been underexplored. In the present study, we experimentally focus on how hyaluronic acid, a protein commonly found in urine, alters the struvite crystallization in aqueous solution and in an artificial urine media. By gradually adding ammonium dihydrogen phosphate to a solution containing magnesium chloride hexahydrate, reactive crystallization is carried out in a well-mixed and thermostated vessel at 37 °C. The resulting struvite crystals are characterized structurally by XRD and FTIR as well as morphologically and in terms of their surface charge. In addition, the thermal decomposition behavior of the struvite with and without hyaluronic acid and released volatile products were simultaneously investigated using a TGA/FTIR system. The average activation energy calculated using the Friedman method was 49.2 ± 5.1 kJ/mol. The results of the kinetic and thermodynamic analyses showed that decomposition of the struvite crystals was endothermic and followed the multiple stage reaction mechanism.
KW - A1. shape parameters
KW - A1. TGA/FTIR
KW - A1. thermal kinetics
KW - B1. crystallization
KW - B1. Struvite
UR - http://www.scopus.com/inward/record.url?scp=85131098375&partnerID=8YFLogxK
U2 - 10.1016/j.jcrysgro.2022.126734
DO - 10.1016/j.jcrysgro.2022.126734
M3 - Article
AN - SCOPUS:85131098375
VL - 592
JO - Journal of Crystal Growth
JF - Journal of Crystal Growth
SN - 0022-0248
M1 - 126734
ER -