TY - JOUR
T1 - Formate Oxidase (FOx) from Aspergillus oryzae
T2 - One Catalyst Enables Diverse H
2
O
2
-Dependent Biocatalytic Oxidation Reactions
AU - Tieves, Florian
AU - Willot, Sébastien Jean Paul
AU - van Schie, Morten Martinus Cornelis Harald
AU - Rauch, Marine Charlène Renée
AU - Younes, Sabry Hamdy Hamed
AU - Zhang, Wuyuan
AU - Dong, Jia Jia
AU - Gomez de Santos, Patricia
AU - Hollmann, Frank
PY - 2019
Y1 - 2019
N2 -
An increasing number of biocatalytic oxidation reactions rely on H
2
O
2
as a clean oxidant. The poor robustness of most enzymes towards H
2
O
2
, however, necessitates more efficient systems for in situ H
2
O
2
generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H
2
O
2
-dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.
AB -
An increasing number of biocatalytic oxidation reactions rely on H
2
O
2
as a clean oxidant. The poor robustness of most enzymes towards H
2
O
2
, however, necessitates more efficient systems for in situ H
2
O
2
generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H
2
O
2
-dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.
KW - biocatalysis
KW - formate oxidase
KW - hydrogen peroxide
KW - oxidation
KW - oxyfunctionalisation
UR - http://www.scopus.com/inward/record.url?scp=85065201966&partnerID=8YFLogxK
U2 - 10.1002/anie.201902380
DO - 10.1002/anie.201902380
M3 - Article
AN - SCOPUS:85065201966
SN - 1433-7851
VL - 58
SP - 7873
EP - 7877
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 23
ER -