Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions

Florian Tieves, Sébastien Jean Paul Willot, Morten Martinus Cornelis Harald van Schie, Marine Charlène Renée Rauch, Sabry Hamdy Hamed Younes, Wuyuan Zhang, Jia Jia Dong, Patricia Gomez de Santos, Frank Hollmann

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)
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An increasing number of biocatalytic oxidation reactions rely on H 2 O 2 as a clean oxidant. The poor robustness of most enzymes towards H 2 O 2 , however, necessitates more efficient systems for in situ H 2 O 2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H 2 O 2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

Original languageEnglish
Pages (from-to)7873-7877
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number23
Publication statusPublished - 2019


  • biocatalysis
  • formate oxidase
  • hydrogen peroxide
  • oxidation
  • oxyfunctionalisation

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