Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Thomas Hollt, Sabine Adam-Klages, More Authors

Research output: Contribution to journalArticleScientificpeer-review

613 Citations (Scopus)


These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.
Original languageEnglish
Pages (from-to)1457-1973
Number of pages517
JournalEuropean Journal of Immunology
Issue number10
Publication statusPublished - 2019


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