Abstract
Osteochondral defect repair with a collagen/collagen-magnesium-hydroxyapatite (Col/Col-Mg-HAp) scaffold has demonstrated good clinical results. However, subchondral bone repair remained suboptimal, potentially leading to damage to the regenerated overlying neocartilage. This study aimed to improve the bone repair potential of this scaffold by incorporating newly developed strontium (Sr) ion enriched amorphous calcium phosphate (Sr-ACP) granules (100–150 μm). Sr concentration of Sr-ACP was determined with ICP-MS at 2.49 ± 0.04 wt%. Then 30 wt% ACP or Sr-ACP granules were integrated into the scaffold prototypes. The ACP or Sr-ACP granules were well embedded and distributed in the collagen matrix demonstrated by micro-CT and scanning electron microscopy/energy dispersive x-ray spectrometry. Good cytocompatibility of ACP/Sr-ACP granules and ACP/Sr-ACP enriched scaffolds was confirmed with in vitro cytotoxicity assays. An overall promising early tissue response and good biocompatibility of ACP and Sr-ACP enriched scaffolds were demonstrated in a subcutaneous mouse model. In a goat osteochondral defect model, significantly more bone was observed at 6 months with the treatment of Sr-ACP enriched scaffolds compared to scaffold-only, in particular in the weight-bearing femoral condyle subchondral bone defect. Overall, the incorporation of osteogenic Sr-ACP granules in Col/Col-Mg-HAp scaffolds showed to be a feasible and promising strategy to improve subchondral bone repair.
Original language | English |
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Article number | 100959 |
Number of pages | 15 |
Journal | Materials Today Bio |
Volume | 25 |
DOIs | |
Publication status | Published - 2024 |
Funding
This work was supported by the European Union's Horizon 2020 research and innovation programme [grant numbers EURONANOMED2017-077 ]; Science Foundation of Ireland [grant Number SFI /16/ENM- ERA /3458]; Ministero della Salute ( IMH ); Stated Education Development Agency SEDA /VIAA; Technology Foundation ( STW ). The authors acknowledge financial support from the European Union's Horizon 2020 research and innovation programme under the grant agreement No. 857287 (BBCE – Baltic Biomaterials Centre of Excellence).This work was supported by the European Union's Horizon 2020 research and innovation programme [grant numbers EURONANOMED2017-077]; Science Foundation of Ireland [grant Number SFI/16/ENM-ERA/3458]; Ministero della Salute (IMH); Stated Education Development Agency SEDA/VIAA; Technology Foundation (STW). The authors acknowledge financial support from the European Union's Horizon 2020 research and innovation programme under the grant agreement No. 857287 (BBCE – Baltic Biomaterials Centre of Excellence).The authors acknowledge Yanto Ridwan for the support in the micro-CT scanning, and Mart Verhoeven and Wouter van de Ven for the support in the micro-CT evaluation. This work was supported through the use of imaging equipment provided bij the Applied Molecular Imaging Erasmus MC facility. The authors acknowledge Christian Poinsot for the support in cytotoxicity assessment.
Keywords
- Amorphous calcium phosphate
- Osteochondral defect
- Regenerative medicine
- Strontium
- Tissue engineering