Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

Chris Sebastiaan Vink; Fernando Jose Calero-Nieto; Xiaonan Wang; Antonio Maglitto; Samanta Antonella Mariani; Wajid Jawaid; Berthold Göttgens; Elaine Dzierzak

doi:10.1016/j.celrep.2020.107627

Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

Chris Sebastiaan Vink, Fernando Jose Calero-Nieto, Xiaonan Wang, Antonio Maglitto, Samanta Antonella Mariani, Wajid Jawaid, Berthold Göttgens, Elaine Dzierzak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

38 Citations (Scopus)

Abstract

Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.

Original language	English
Article number	107627
Number of pages	18
Journal	Cell Reports
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2020.107627
Publication status	Published - 2020
Externally published	Yes

Keywords

AGM
CD27
development
embryo
functional identity
Gata2
hematopoietic stem cell
heterogeneity
intra-aortic hematopoietic clusters
single-cell transcriptome
transplantation

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10.1016/j.celrep.2020.107627

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title = "Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells",

abstract = "Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.",

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AU - Vink, Chris Sebastiaan

AU - Calero-Nieto, Fernando Jose

AU - Wang, Xiaonan

AU - Maglitto, Antonio

AU - Mariani, Samanta Antonella

AU - Jawaid, Wajid

AU - Göttgens, Berthold

AU - Dzierzak, Elaine

PY - 2020

Y1 - 2020

N2 - Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.

AB - Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.

KW - AGM

KW - CD27

KW - development

KW - embryo

KW - functional identity

KW - Gata2

KW - hematopoietic stem cell

KW - heterogeneity

KW - intra-aortic hematopoietic clusters

KW - single-cell transcriptome

KW - transplantation

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DO - 10.1016/j.celrep.2020.107627

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SN - 2211-1247

VL - 31

JO - Cell Reports

JF - Cell Reports

IS - 6

M1 - 107627

ER -

Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

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Keywords

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