TY - JOUR
T1 - Leloir Glycosyltransferases in Applied Biocatalysis
T2 - A Multidisciplinary Approach
AU - Mestrom, Luuk
AU - Przypis, Marta
AU - Kowalczykiewicz, Daria
AU - Pollender, André
AU - Kumpf, Antje
AU - Marsden, Stefan R.
AU - Szymańska, Katarzyna
AU - Hanefeld, Ulf
AU - Hagedoorn, Peter Leon
AU - More Authors, null
PY - 2019
Y1 - 2019
N2 - Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio- and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations.
AB - Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio- and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations.
KW - applied biocatalysis
KW - carbohydrate
KW - chemoenzymatic synthesis
KW - enzyme cascades
KW - glycosyltransferase
KW - Leloir
KW - nucleotide
KW - sugar chemistry
UR - http://www.scopus.com/inward/record.url?scp=85074169557&partnerID=8YFLogxK
U2 - 10.3390/ijms20215263
DO - 10.3390/ijms20215263
M3 - Review article
C2 - 31652818
AN - SCOPUS:85074169557
SN - 1422-0067
VL - 20
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 21
M1 - 5263
ER -