TY - JOUR
T1 - Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software
AU - Kaiser, Matthias
AU - Jug, Florian
AU - Julou, Thomas
AU - Deshpande, Siddharth
AU - Pfohl, Thomas
AU - Silander, Olin K.
AU - Myers, Gene
AU - Van Nimwegen, Erik
PY - 2018
Y1 - 2018
N2 - Much is still not understood about how gene regulatory interactions control cell fate decisions in single cells, in part due to the difficulty of directly observing gene regulatory processes in vivo. We introduce here a novel integrated setup consisting of a microfluidic chip and accompanying analysis software that enable long-term quantitative tracking of growth and gene expression in single cells. The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells. The Mother Machine Analyzer (MoMA) software achieves unprecedented accuracy in segmenting and tracking cells, and streamlines high-throughput curation with a novel leveraged editing procedure. We demonstrate the power of the method by uncovering several novel features of an iconic gene regulatory program: the induction of Escherichia coli's lac operon in response to a switch from glucose to lactose.
AB - Much is still not understood about how gene regulatory interactions control cell fate decisions in single cells, in part due to the difficulty of directly observing gene regulatory processes in vivo. We introduce here a novel integrated setup consisting of a microfluidic chip and accompanying analysis software that enable long-term quantitative tracking of growth and gene expression in single cells. The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells. The Mother Machine Analyzer (MoMA) software achieves unprecedented accuracy in segmenting and tracking cells, and streamlines high-throughput curation with a novel leveraged editing procedure. We demonstrate the power of the method by uncovering several novel features of an iconic gene regulatory program: the induction of Escherichia coli's lac operon in response to a switch from glucose to lactose.
UR - http://resolverlink.tudelft.nl/uuid:e1c43d42-722d-436e-89b0-7067a072cf8d
UR - http://www.scopus.com/inward/record.url?scp=85040796069&partnerID=8YFLogxK
U2 - 10.1038/s41467-017-02505-0
DO - 10.1038/s41467-017-02505-0
M3 - Article
C2 - 29335514
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 212
ER -