TY - JOUR
T1 - Open-culture biotechnological process for triacylglycerides and polyhydroxyalkanoates recovery from industrial waste fish oil under saline conditions
AU - Argiz, Lucia
AU - Gonzalez-Cabaleiro, Rebeca
AU - Correa-Galeote, David
AU - Val del Rio, Angeles
AU - Mosquera-Corral, Anuska
PY - 2021
Y1 - 2021
N2 - Industrial waste fish oil streams contain high concentrations of medium and long-chain fatty acids suitable to produce value-added compounds. However, to process them dilution is required, and the water produced in the fish-canning industry commonly contains high salinity, which might limit its reuse as a dilution stream. Although NaCl is well-known to negatively affect biological activity, its effect on triacylglycerides (TAG) and polyhydroxyalkanoates (PHA) storage has not been well studied yet. Here, it was explored if intracellular TAG and PHA production can be efficient under saline conditions (10 g NaCl/L). For that purpose, waste fish oil was valorised using a mixed microbial culture (MMC) in a two-stage process (culture selection plus accumulation). Results showed that salinity influenced not only the activity but the structure of the microbial communities developed in the bioreactors. The bacterial genera Acinetobacter and Rhizobium and the mold Candida glaebosa clade were observed as the storing microorganisms which abundance increased under saline conditions whereas Dipodascus and Mortierella notably decreased. Nonetheless, despite the osmotic stress, promising results were obtained and maximum intracellular accumulations of 54.2 wt% (TAG:PHA = 28:72, 0.131 CmmolTAG/CmmolS, 0.303 CmmolPHA/CmmolS) and 50.9 wt% (TAG:PHA = 63:37, 0.291 CmmolTAG/CmmolS, 0.114 CmmolPHA/CmmolS) were observed when PHA and TAG were preferentially stored, respectively.
AB - Industrial waste fish oil streams contain high concentrations of medium and long-chain fatty acids suitable to produce value-added compounds. However, to process them dilution is required, and the water produced in the fish-canning industry commonly contains high salinity, which might limit its reuse as a dilution stream. Although NaCl is well-known to negatively affect biological activity, its effect on triacylglycerides (TAG) and polyhydroxyalkanoates (PHA) storage has not been well studied yet. Here, it was explored if intracellular TAG and PHA production can be efficient under saline conditions (10 g NaCl/L). For that purpose, waste fish oil was valorised using a mixed microbial culture (MMC) in a two-stage process (culture selection plus accumulation). Results showed that salinity influenced not only the activity but the structure of the microbial communities developed in the bioreactors. The bacterial genera Acinetobacter and Rhizobium and the mold Candida glaebosa clade were observed as the storing microorganisms which abundance increased under saline conditions whereas Dipodascus and Mortierella notably decreased. Nonetheless, despite the osmotic stress, promising results were obtained and maximum intracellular accumulations of 54.2 wt% (TAG:PHA = 28:72, 0.131 CmmolTAG/CmmolS, 0.303 CmmolPHA/CmmolS) and 50.9 wt% (TAG:PHA = 63:37, 0.291 CmmolTAG/CmmolS, 0.114 CmmolPHA/CmmolS) were observed when PHA and TAG were preferentially stored, respectively.
KW - Lipids
KW - Mixed microbial culture
KW - Polyhydroxyalkanoates
KW - Salinity
KW - Triacylglycerides
UR - http://www.scopus.com/inward/record.url?scp=85104747889&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2021.118805
DO - 10.1016/j.seppur.2021.118805
M3 - Article
AN - SCOPUS:85104747889
SN - 1383-5866
VL - 270
SP - 11
JO - Separation and Purification Technology
JF - Separation and Purification Technology
M1 - 118805
ER -