Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Bent O. Petersen; Margit Sára; Christoph Mader; Harald F. Mayer; Uwe B. Sleytr; Martin Pabst; Michael Puchberger; Eberhard Krause; Andreas Hofinger; null More Authors

doi:10.1016/j.carres.2008.03.029

Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Bent O. Petersen, Margit Sára, Christoph Mader, Harald F. Mayer, Uwe B. Sleytr, Martin Pabst, Michael Puchberger, Eberhard Krause, Andreas Hofinger, More Authors

Research output: Contribution to journal › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide-liberated by HF-treatment from the cell wall-was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:{A figure is presented}.

Original language	English
Pages (from-to)	1346-1358
Journal	Carbohydrate Research
Volume	343
Issue number	8
DOIs	https://doi.org/10.1016/j.carres.2008.03.029
Publication status	Published - 2008
Externally published	Yes

Keywords

Geobacillus
Mass spectrometry
NMR spectroscopy
Polysaccharide structure
Secondary cell wall polymer

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10.1016/j.carres.2008.03.029

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title = "Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2",

abstract = "The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide-liberated by HF-treatment from the cell wall-was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:{A figure is presented}.",

keywords = "Geobacillus, Mass spectrometry, NMR spectroscopy, Polysaccharide structure, Secondary cell wall polymer",

author = "Petersen, {Bent O.} and Margit S{\'a}ra and Christoph Mader and Mayer, {Harald F.} and Sleytr, {Uwe B.} and Martin Pabst and Michael Puchberger and Eberhard Krause and Andreas Hofinger and {More Authors}",

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language = "English",

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T1 - Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

AU - Petersen, Bent O.

AU - Sára, Margit

AU - Mader, Christoph

AU - Mayer, Harald F.

AU - Sleytr, Uwe B.

AU - Pabst, Martin

AU - Puchberger, Michael

AU - Krause, Eberhard

AU - Hofinger, Andreas

AU - More Authors, null

PY - 2008

Y1 - 2008

N2 - The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide-liberated by HF-treatment from the cell wall-was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:{A figure is presented}.

AB - The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-mannose, and 2-acetamido-2-deoxy-d-mannuronic acid. The primary structure of the acid-degraded polysaccharide-liberated by HF-treatment from the cell wall-was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-α-d-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-d-glucopyranosyl units:{A figure is presented}.

KW - Geobacillus

KW - Mass spectrometry

KW - NMR spectroscopy

KW - Polysaccharide structure

KW - Secondary cell wall polymer

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DO - 10.1016/j.carres.2008.03.029

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JO - Carbohydrate Research

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IS - 8

ER -

Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

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