TY - JOUR
T1 - Surfing the wave of oxyfunctionalization chemistry by engineering fungal unspecific peroxygenases
AU - Beltrán-Nogal, Alejandro
AU - Sánchez-Moreno, Israel
AU - Méndez-Sánchez, Daniel
AU - Gómez de Santos, Patricia
AU - Hollmann, Frank
AU - Alcalde, Miguel
PY - 2022
Y1 - 2022
N2 - The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown of C–H oxyfunctionalization biocatalysts. Although their limited availability and development has hindered their incorporation into industry, the conjunction of directed evolution and computational design is approaching UPOs to practical applications. In this review, we will address the most recent advances in UPO engineering, both of the long and short UPO families, while discussing the future prospects in this fast-moving field of research.
AB - The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown of C–H oxyfunctionalization biocatalysts. Although their limited availability and development has hindered their incorporation into industry, the conjunction of directed evolution and computational design is approaching UPOs to practical applications. In this review, we will address the most recent advances in UPO engineering, both of the long and short UPO families, while discussing the future prospects in this fast-moving field of research.
UR - http://www.scopus.com/inward/record.url?scp=85125393313&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2022.102342
DO - 10.1016/j.sbi.2022.102342
M3 - Review article
AN - SCOPUS:85125393313
SN - 0959-440X
VL - 73
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
M1 - 102342
ER -