TY - JOUR
T1 - Syntrophy between fermentative and purple phototrophic bacteria to treat and valorize carbohydrate-rich wastewaters
AU - Cerruti, Marta
AU - Crosset-Perrotin, Guillaume
AU - Ananth, Mythili
AU - Rombouts, Julius Laurens
AU - Weissbrodt, David G.
PY - 2023
Y1 - 2023
N2 - Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor regimes (batch/chemostat) and illumination modes (continuous infrared light, dark, or light/dark cycles) on glucose conversions and process ecology of the interactions between FCB and PPB in mixed cultures. In batch, FCB (>80 % of sequencing read counts) outcompeted PPB, under any light conditions. In chemostat under continuous and alternating irradiance, three FCB populations were enriched (>70 %), while Rhodobacteraceae (PPB) made 30 % of the community. Glucose fermentation products were linked to the dominant FCB. Continuous culturing helped maintaining FCB and PPB in syntrophy: PPB grew on glucose metabolites produced by FCB. Engineering the association between FCB and PPB in mixed-culture processes can help to treat and valorize carbohydrate-rich aqueous waste.
AB - Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor regimes (batch/chemostat) and illumination modes (continuous infrared light, dark, or light/dark cycles) on glucose conversions and process ecology of the interactions between FCB and PPB in mixed cultures. In batch, FCB (>80 % of sequencing read counts) outcompeted PPB, under any light conditions. In chemostat under continuous and alternating irradiance, three FCB populations were enriched (>70 %), while Rhodobacteraceae (PPB) made 30 % of the community. Glucose fermentation products were linked to the dominant FCB. Continuous culturing helped maintaining FCB and PPB in syntrophy: PPB grew on glucose metabolites produced by FCB. Engineering the association between FCB and PPB in mixed-culture processes can help to treat and valorize carbohydrate-rich aqueous waste.
KW - Chemoorganoheterotrophs
KW - Mixed-culture fermentation
KW - Photoorganoheterotrophs
KW - Resource recovery
KW - Syntrophy
UR - http://www.scopus.com/inward/record.url?scp=85151784156&partnerID=8YFLogxK
U2 - 10.1016/j.biteb.2023.101348
DO - 10.1016/j.biteb.2023.101348
M3 - Article
AN - SCOPUS:85151784156
VL - 22
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
M1 - 101348
ER -