The Development of Tungsten Biochemistry-A Personal Recollection

Wilfred R. Hagen

doi:10.3390/molecules28104017

The Development of Tungsten Biochemistry-A Personal Recollection

Wilfred R. Hagen

BT/Biocatalysis

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Abstract

The development of tungsten biochemistry is sketched from the viewpoint of personal participation. Following its identification as a bio-element, a catalogue of genes, enzymes, and reactions was built up. EPR spectroscopic monitoring of redox states was, and remains, a prominent tool in attempts to understand tungstopterin-based catalysis. A paucity of pre-steady-state data remains a hindrance to overcome to this day. Tungstate transport systems have been characterized and found to be very specific for W over Mo. Additional selectivity is presented by the biosynthetic machinery for tungstopterin enzymes. Metallomics analysis of hyperthermophilic archaeon Pyrococcus furiosus indicates a comprehensive inventory of tungsten proteins.

Original language	English
Article number	4017
Number of pages	18
Journal	Molecules (Basel, Switzerland)
Volume	28
Issue number	10
DOIs	https://doi.org/10.3390/molecules28104017
Publication status	Published - 2023

Keywords

aldehyde oxidoreductase
EPR
metallomics
Pyrococcus furiosus
transport
tungsten
tungstoenzyme
tungstopterin

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10.3390/molecules28104017

molecules-28-04017Final published version, 3.49 MBLicence: CC BY

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abstract = "The development of tungsten biochemistry is sketched from the viewpoint of personal participation. Following its identification as a bio-element, a catalogue of genes, enzymes, and reactions was built up. EPR spectroscopic monitoring of redox states was, and remains, a prominent tool in attempts to understand tungstopterin-based catalysis. A paucity of pre-steady-state data remains a hindrance to overcome to this day. Tungstate transport systems have been characterized and found to be very specific for W over Mo. Additional selectivity is presented by the biosynthetic machinery for tungstopterin enzymes. Metallomics analysis of hyperthermophilic archaeon Pyrococcus furiosus indicates a comprehensive inventory of tungsten proteins.",

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AB - The development of tungsten biochemistry is sketched from the viewpoint of personal participation. Following its identification as a bio-element, a catalogue of genes, enzymes, and reactions was built up. EPR spectroscopic monitoring of redox states was, and remains, a prominent tool in attempts to understand tungstopterin-based catalysis. A paucity of pre-steady-state data remains a hindrance to overcome to this day. Tungstate transport systems have been characterized and found to be very specific for W over Mo. Additional selectivity is presented by the biosynthetic machinery for tungstopterin enzymes. Metallomics analysis of hyperthermophilic archaeon Pyrococcus furiosus indicates a comprehensive inventory of tungsten proteins.

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KW - EPR

KW - metallomics

KW - Pyrococcus furiosus

KW - transport

KW - tungsten

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KW - tungstopterin

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The Development of Tungsten Biochemistry-A Personal Recollection

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