Toward Kilogram-Scale Peroxygenase-Catalyzed Oxyfunctionalization of Cyclohexane

Thomas Hilberath, Remco Van Oosten, Juliet Victoria, Hugo Brasselet, Miguel Alcalde, John M. Woodley, Frank Hollmann*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)


Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using AaeUPO from Agrocybe aegerita and simple H2O2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h-1, approx. 15 g L-1 h-1) were achieved. Current limitations of the proposed biooxidation system have been identified paving the way for future improvements and implementation.

Original languageEnglish
Pages (from-to)1384-1389
JournalOrganic Process Research and Development
Issue number7
Publication statusPublished - 2023


  • biocatalysis
  • bulk chemical
  • cyclohexane
  • oxyfunctionalization
  • peroxygenase
  • upscaling


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