Towards the development of a droplet micro-reactor for industrial relevant screening in biotechnology

Kartik Totlani, Thorben de Reisse, Maxime Bisschops, Walter van Gulik, Michiel Kreutzer, Volkert van Steijn*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

Abstract

We report the development of a droplet-based microfluidic device, which enables long term culturing of microorganisms inside water in oil microdroplets under semi-continuous conditions. Firstly, a microdroplet containing yeast cells is trapped on chip. After an initial incubation phase, this cell laden droplet is coalesced with smaller nutrient droplets to enable cultivation and time-lapsing under semi-continuous conditions. We demonstrate a proof-of-principle study on a sequential batch experiment, which lays a sound foundation for screening of microorganisms under semi-continuous conditions.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1005-1006
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period27/10/1931/10/19

Keywords

  • Cell growth
  • Coalescence
  • Microdroplets
  • Microfluidics
  • Nutrient feeding
  • Screening

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