Abstract
We report the development of a droplet-based microfluidic device, which enables long term culturing of microorganisms inside water in oil microdroplets under semi-continuous conditions. Firstly, a microdroplet containing yeast cells is trapped on chip. After an initial incubation phase, this cell laden droplet is coalesced with smaller nutrient droplets to enable cultivation and time-lapsing under semi-continuous conditions. We demonstrate a proof-of-principle study on a sequential batch experiment, which lays a sound foundation for screening of microorganisms under semi-continuous conditions.
| Original language | English |
|---|---|
| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1005-1006 |
| Number of pages | 2 |
| ISBN (Electronic) | 978-1-7334-1900-0 |
| ISBN (Print) | 978-1-7138-1852-6 |
| Publication status | Published - 2019 |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 |
Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|---|
| Country/Territory | Switzerland |
| City | Basel |
| Period | 27/10/19 → 31/10/19 |
Keywords
- Cell growth
- Coalescence
- Microdroplets
- Microfluidics
- Nutrient feeding
- Screening