Abstract
Bovine serum albumin (BSA) and casein (CAS) were used in batch tests to compare the protein degradation in the presence and absence of carbohydrates and volatile fatty acids (VFAs). The modified Gompertz model was applied to estimate reaction rates. The results showed that deamination was the rate-limiting step, with a rate ranging between 2.7 and 12.7 mgN·h−1. Higher protein structural complexity negatively affected protein hydrolysis, deamination, and methanogenesis by a factor of 1.6–3.8; whereas a higher protein concentration improved the conversion rates. A carbohydrate:protein COD ratio of 1 improved the hydrolysis rate of BSA from 26 mg·h−1 to 45 mg·h−1, and that of CAS from 98 mg·h−1 to 157 mg·h−1; whereas the deamination rate slightly decreased from 2.7 mg N·h−1 to 2.5 mg N·h−1 and from 6.0 mg N·h−1 to 5.6 mg N·h−1. Additionally, an initial VFAs:protein COD ratio of 1 decreased the CAS deamination rate by 17 %.
Original language | English |
---|---|
Article number | 101501 |
Number of pages | 9 |
Journal | Bioresource Technology Reports |
Volume | 22 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-careOtherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Keywords
- Anaerobic digestion
- Carbohydrates
- Modified Gompertz models
- Protein
- Rate-limiting step
- Volatile fatty acids