Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

A.C.A. van Aalst

doi:10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe

Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

A.C.A. van Aalst

BT/Industriele Microbiologie

Research output: Thesis › Dissertation (TU Delft)

52 Downloads (Pure)

Abstract

The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	Delft University of Technology
Supervisors/Advisors	Pronk, J.T., Supervisor Daran, J.G., Supervisor
Thesis sponsors	DSM
Award date	2 Oct 2023
Print ISBNs	978-94-6483-352-2
DOIs	https://doi.org/10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe
Publication status	Published - 2023

Keywords

Redox engineering
Ethanol production
Saccharomyces cerevisiae
co-culture
Metabolic engineering
anaerobic fermentation
Electrons
industrial biotechnology

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10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe

Aafke van Aalst_dissertationFinal published version, 60.8 MBLicence: CC BY-ND

3 Article

Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes
van Aalst, A. C. A., Jansen, M. L. A., Mans, R. & Pronk, J. T., 2023, In: Biotechnology for Biofuels and Bioproducts. 16, 1, 17 p., 81.
Research output: Contribution to journal › Article › Scientific › peer-review

Open Access
File
3 Citations (Scopus)

41 Downloads (Pure)
An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae
van Aalst, A. C. A., Mans, R. & Pronk, J. T., 2022, In: Biotechnology for Biofuels and Bioproducts. 15, 1, 112.
Research output: Contribution to journal › Article › Scientific › peer-review

Open Access
File
6 Citations (Scopus)

29 Downloads (Pure)
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production
van Aalst, A. C. A., de Valk, S. C., van Gulik, W. M., Jansen, M. L. A., Pronk, J. T. & Mans, R., 2022, In: Synthetic and Systems Biotechnology. 7, 1, p. 554-566
Research output: Contribution to journal › Article › Scientific › peer-review

Open Access
File
13 Citations (Scopus)

176 Downloads (Pure)

Cite this

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title = "Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast",

abstract = "The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.",

keywords = "Redox engineering, Ethanol production, Saccharomyces cerevisiae, co-culture, Metabolic engineering, anaerobic fermentation, Electrons, industrial biotechnology",

author = "{van Aalst}, A.C.A.",

year = "2023",

doi = "10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe",

language = "English",

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type = "Dissertation (TU Delft)",

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T1 - Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

AU - van Aalst, A.C.A.

PY - 2023

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N2 - The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.

AB - The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.

KW - Redox engineering

KW - Ethanol production

KW - Saccharomyces cerevisiae

KW - co-culture

KW - Metabolic engineering

KW - anaerobic fermentation

KW - Electrons

KW - industrial biotechnology

U2 - 10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe

DO - 10.4233/uuid:9a149309-2339-40c5-9151-032d70b91dbe

M3 - Dissertation (TU Delft)

SN - 978-94-6483-352-2

ER -

Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

Abstract

Keywords

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Research output

Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes

An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae

Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Cite this