Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast

Research output: ThesisDissertation (TU Delft)

52 Downloads (Pure)


The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Delft University of Technology
  • Pronk, J.T., Supervisor
  • Daran, J.G., Supervisor
Thesis sponsors
Award date2 Oct 2023
Print ISBNs978-94-6483-352-2
Publication statusPublished - 2023


  • Redox engineering
  • Ethanol production
  • Saccharomyces cerevisiae
  • co-culture
  • Metabolic engineering
  • anaerobic fermentation
  • Electrons
  • industrial biotechnology


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