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Research Output

2020

Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to Hosts

de Jonge, P. A., von Meijenfeldt, F. A. B., Costa, A. R., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 2020, In : iScience. 23, 9, 101439.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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1 Downloads (Pure)

An educational guide for nanopore sequencing in the classroom

Salazar, A. N., Nobrega, F. L., Anyansi, C., Aparicio-Maldonado, C., Costa, A. R., Haagsma, A. C., Hiralal, A., Mahfouz, A., McKenzie, R. E., van Rossum, T., Brouns, S. J. J. & Abeel, T., 2020, In : PLoS Computational Biology. 16, 1, p. e1007314 7 p., e1007314.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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1 Citation (Scopus)
81 Downloads (Pure)

Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., Brocken, D. J. W., Hohlbein, J. & Brouns, S. J. J., 2020, In : Molecular Cell. 77, 1, p. 39-50.e10

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Mining moon & mars with microbes: Biological approaches to extract iron from Lunar and Martian regolith

Volger, R., Pettersson, G. M., Brouns, S. J. J., Rothschild, L. J., Cowley, A. & Lehner, B. A. E., 2020, In : Planetary and Space Science. 184, 9 p., 104850.

Research output: Contribution to journalArticleScientificpeer-review

Selective loading and processing of prespacers for precise CRISPR adaptation

Kim, S., Loeff, L., Colombo, S., Jergic, S., Brouns, S. J. J. & Joo, C., 2020, In : Nature. 579, 7797, p. 141-145 5 p.

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)
2019

Cas4-Cas1 fusions drive efficient PAM selection and control CRISPR adaptation

Almendros, C., Nobrega, F. L., McKenzie, R. E. & Brouns, S. J. J., 2019, In : Nucleic Acids Research. 47, 10, p. 5223-5230

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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6 Citations (Scopus)
56 Downloads (Pure)

Coacervate-based detection of a compound of interest

Brouns, S., Reese, L. & Spoelstra, K., 2019, IPC No. C12Q, Priority date 20 Oct 2017, Priority No. NL2019775

Research output: Patent

Open Access
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3 Citations (Scopus)
48 Downloads (Pure)

Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

Lehner, B. A. E., Janssen, V. A. E. C., Spiesz, E. M., Benz, D., Brouns, S. J. J., Meyer, A. S. & van der Zant, H. S. J., 2019, In : ChemistryOpen. 8, 7, p. 888-895

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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3 Citations (Scopus)
49 Downloads (Pure)

CRISPR-Cas Systems Reduced to a Minimum

Almendros, C., Kieper, S. N. & Brouns, S. J. J., 21 Feb 2019, In : Molecular Cell. 73, 4, p. 641-642

Research output: Contribution to journalReview articleScientificpeer-review

End-to-end mission design for microbial ISRU activities as preparation for a moon village

Lehner, B. A. E., Schlechten, J., Filosa, A., Canals Pou, A., Mazzotta, D. G., Spina, F., Tjon, S. Y., Meyer, A. S., Brouns, S. J. J. & More Authors, 2019, In : Acta Astronautica. 162, p. 216-226

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Shah, S. A. & More Authors, 2019, In : Nature Reviews Microbiology. 17 p.

Research output: Contribution to journalReview articleScientificpeer-review

42 Citations (Scopus)

Evolution of BACON Domain Tandem Repeats in crAssphage and Novel Gut Bacteriophage Lineages

Jonge, P. A. D., Meijenfeldt, F. A. B. V., Rooijen, L. E. V., Brouns, S. J. J. & Dutilh, B. E., 2019, In : Viruses. 11, 12, 17 p., v11121085.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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3 Citations (Scopus)
20 Downloads (Pure)

Global phylogeography and ancient evolution of the widespread human gut virus crAssphage

Edwards, R. A., Vega, A. A., Dutilh, B. E., Norman, H. M., Ohaeri, M. C., Levi, K., Brouns, S., de Jonge, P., Luzia De Nóbrega, F. & More Authors, 2019, In : Nature Microbiology. 4, 10, p. 1727-1736

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Kennedy, R., Brouns, S. J. J. & More Authors, 2019, In : Nature Biotechnology. 37, 12, p. 1471-1477 7 p.

Research output: Contribution to journalLetterScientificpeer-review

9 Citations (Scopus)

Molecular and Evolutionary Determinants of Bacteriophage Host Range

de Jonge, P. A., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 1 Jan 2019, In : Trends in Microbiology. 27, 1, p. 51-63 13 p.

Research output: Contribution to journalArticleScientificpeer-review

34 Citations (Scopus)

Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation

Musharova, O., Sitnik, V., Vlot, M., Savitskaya, E., Datsenko, K. A., Krivoy, A., Fedorov, I., Semenova, E., Brouns, S. J. J. & Severinov, K., 2019, In : Molecular Microbiology. 111, 6, p. 1558-1570

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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4 Citations (Scopus)
50 Downloads (Pure)

Using CAPTURE to detect spacer acquisition in native CRISPR arrays

McKenzie, R. E., Almendros, C., Vink, J. N. A. & Brouns, S. J. J., 2019, In : Nature Protocols. 14, 3, p. 976-990

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Visualisation of dCas9 target search in vivo using an open-microscopy framework

Martens, K., van Beljouw, S. P. B., van der Els, S., Vink, J. N. A., Baas, S., Vogelaar, G. A., Brouns, S. J. J., van Baarlen, P., Kleerebezem, M. & Hohlbein, J., 2019, In : Nature Communications. 10, 1, 11 p., 3552.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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7 Citations (Scopus)
48 Downloads (Pure)
2018

Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes

Vlot, M., Houkes, J., Lochs, S. J. A., Swarts, D. C., Zheng, P., Kunne, T., Mohanraju, P., Anders, C., Jinek, M., Van Der Oost, J., Dickman, M. J. & Brouns, S. J. J., 2018, In : Nucleic Acids Research. 46, 2, p. 873-885 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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14 Citations (Scopus)
35 Downloads (Pure)

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

Kieper, S. N., Almendros, C., Behler, J., McKenzie, R. E., Luzia De Nóbrega, F., Haagsma, A. C., Vink, J. N. A., Hess, W. R. & Brouns, S. J. J., 2018, In : Cell Reports. 22, 13, p. 3377-3384 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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37 Citations (Scopus)
16 Downloads (Pure)

Complete genome sequence of the Escherichia coli phage Ayreon

Vlot, M., Nobrega, F. L., Wong, C. F. A., Liu, Y. & Brouns, S. J. J., 2018, In : Genome Announcements. 6, 2, e01354-17.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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19 Downloads (Pure)

Complete genome sequences of two T4-like Escherichia coli bacteriophages

Costa, A. R., Brouns, S. J. J. & Nobrega, F. L., 1 Jun 2018, In : Genome Announcements. 6, 26, e00586-18.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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1 Citation (Scopus)
22 Downloads (Pure)

Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps

Loeff, L., Brouns, S. J. J. & Joo, C., 2018, In : Molecular Cell. 70, 3, p. 385-394.e3

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Role of nucleotide identity in effective CRISPR target escape mutations

Künne, T., Zhu, Y., da Silva, F., Konstantinides, N., McKenzie, R. E., Jackson, R. N. & Brouns, S. J., 2018, In : Nucleic Acids Research. 46, 19, p. 10395-10404

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Targeting mechanisms of tailed bacteriophages

Nobrega, F. L., Vlot, M., de Jonge, P. A., Dreesens, L. L., Beaumont, H. J. E., Lavigne, R., Dutilh, B. E. & Brouns, S. J. J., 2018, In : Nature Reviews Microbiology. 14 p.

Research output: Contribution to journalArticleScientificpeer-review

52 Citations (Scopus)
2017

CRISPR-Cas: Adapting to change

Jackson, S. A., McKenzie, R. E., Fagerlund, R. D., Kieper, S. N., Fineran, P. C. & Brouns, S. J. J., 7 Apr 2017, In : Science. 356, 6333, p. 1-9 9 p., eaal5056.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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127 Citations (Scopus)
87 Downloads (Pure)
2016

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

Künne, T., Kieper, S. N., Bannenberg, J. W., Vogel, A. I. M., Miellet, W. R., Klein, M., Depken, M., Suarez-Diez, M. & Brouns, S. J. J., 1 Sep 2016, In : Molecular Cell. 63, 5, p. 852-864 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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61 Citations (Scopus)
147 Downloads (Pure)
2015

Two distinct DNA binding modes guide dual roles of a CRISPR-cas protein complex

Blosser, T. R., Loeff, L., Westra, E. R., Vlot, M., Künne, T., Sobota, M., Dekker, C., Brouns, S. J. J. & Joo, C., 2015, In : Molecular Cell. 58, 1, p. 60-70

Research output: Contribution to journalArticleScientificpeer-review

65 Citations (Scopus)