S.J.J. Brouns

Search results

• 2022

  Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA

  Koopal, B., Potocnik, A., Mutte, S. K., Aparicio-Maldonado, C., Lindhoud, S., Vervoort, J. J. M., Brouns, S. J. J. & Swarts, D. C., 2022, In: Cell. 185, 9, p. 1471-1486 16 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Argonaute Proteins 100%
  • Cell Death 68%
  • DNA 35%
  • NAD(P) 35%
  • NAD 33%
  5 Citations (Scopus)

  8 Downloads (Pure)

• Single cell variability of CRISPR-Cas interference and adaptation

  McKenzie, R. E., Keizer, E. M., Vink, J. N. A., van Lopik, J., Büke, F., Kalkman, V., Fleck, C., Tans, S. J. & Brouns, S. J. J., 2022, In: Molecular Systems Biology. 18, 4, 18 p., e10680.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clearance 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 89%
  • Interference 89%
  • Bacteria 86%
  • crossover interference 80%
  1 Citation (Scopus)

  7 Downloads (Pure)

• Structural basis for broad anti-phage immunity by DISARM

  Bravo, J. P. K., Aparicio-Maldonado, C., Nobrega, F. L., Brouns, S. J. J. & Taylor, D. W., 2022, In: Nature Communications. 13, 1, 11 p., 2987.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Antiphage 100%
  • immunity 58%
  • Islands 54%
  • Bacteriophages 49%
  • deoxyribonucleic acid 45%
  3 Downloads (Pure)
• 2021

  Cas4-Cas1 Is a Protospacer Adjacent Motif-Processing Factor Mediating Half-Site Spacer Integration during CRISPR Adaptation

  Kieper, S. N., Almendros, C., Haagsma, A. C., Barendregt, A., Heck, A. J. R. & Brouns, S. J. J., 2021, In: CRISPR Journal. 4, 4, p. 536-548

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • CRISPR-Cas Systems 69%
  • Archaea 26%
  • Cues 18%
  • Immunization 18%

• CRISPR-based DNA and RNA detection with liquid-liquid phase separation

  Spoelstra, W. K., Jacques, J. M., Gonzalez-Linares, R., Nobrega, F. L., Haagsma, A. C., Dogterom, M., Meijer, D. H., Idema, T., Brouns, S. J. J. & Reese, L., 2021, In: Biophysical Journal. 120, 7, p. 1198-1209 12 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Nucleic Acids 85%
  • Polynucleotides 77%
  • RNA 60%
  • polycations 50%
  8 Citations (Scopus)

• Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae

  Bonilla, E., Costa, A. R., Van Den Berg, D. F., Van Rossum, T., Hagedoorn, S., Walinga, H., Xiao, M., Haas, P. J., Brouns, S. J. J. & More Authors, 2021, In: DNA Research. 28, 4, 11 p., dsab013.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Klebsiella pneumoniae 100%
  • Bacteriophages 92%
  • Genome 23%
  • Klebsiella Infections 13%
  • Phage Therapy 13%
  2 Citations (Scopus)

  68 Downloads (Pure)

• Iron can be microbially extracted from Lunar and Martian regolith simulants and 3D printed into tough structural materials

  Castelein, S. M., Aarts, T. F., Schleppi, J., Hendrikx, R., Böttger, A. J., Benz, D., Brouns, S. J. J., Meyer, A. S., Lehner, B. A. E. & More Authors, 2021, In: PLoS ONE. 16, April, 21 p., e0249962.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Iron 100%
  • Shewanella 61%
  • Bacteria 54%
  • Compressive Strength 53%
  • Space Flight 51%
  3 Citations (Scopus)

  49 Downloads (Pure)

• Mechanism for Cas4-assisted directional spacer acquisition in CRISPR–Cas

  Hu, C., Almendros, C., Nam, K. H., Costa, A. R., Vink, J. N. A., Haagsma, A. C., Bagde, S. R., Brouns, S. J. J. & Ke, A., 2021, In: Nature. 598, 7881, p. 515-520 6 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Geobacter 30%
  • Interspersed Repetitive Sequences 24%
  • Guide RNA 24%
  • Integrases 22%
  5 Citations (Scopus)

• PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems

  Vink, J. N. A., Baijens, J. H. L. & Brouns, S. J. J., 2021, In: Genome biology. 22, 1, 25 p., 281.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR sequences 100%
  • CRISPR-Cas Systems 95%
  • CRISPR-Cas systems 85%
  • Clustered Regularly Interspaced Short Palindromic Repeats 68%
  • targeting 59%
  4 Citations (Scopus)

  9 Downloads (Pure)

• SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

  Steens, J. A., Zhu, Y., Taylor, D. W., Bravo, J. P. K., Prinsen, S. H. P., Schoen, C. D., Keijser, B. J. F., Ossendrijver, M., Brouns, S. J. J. & More Authors, 2021, In: Nature Communications. 12, 1, 12 p., 5033.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • 2',5'-oligoadenylate 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 76%
  • Ribonucleases 74%
  • RNA Cleavage 65%
  • activation 52%
  9 Citations (Scopus)

  25 Downloads (Pure)

• The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

  van Beljouw, S. P. B., van Eijkeren-Haagsma, A. C., Rodriguez Molina, A., van den Berg, D. F., Vink, J. N. A. & Brouns, S. J. J., 2021, In: Science (New York, N.Y.). 373, 6561, p. 1349-1353

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Endonucleases 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 95%
  • Peptide Hydrolases 70%
  • RNA 57%
  • Immunity 45%
  10 Citations (Scopus)

  129 Downloads (Pure)
• 2020

  Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to Hosts

  de Jonge, P. A., von Meijenfeldt, F. A. B., Costa, A. R., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 2020, In: iScience. 23, 9, 101439.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Adsorption 100%
  • Bacteriophages 91%
  • Waste Water 11%
  • Electrophoresis 8%
  • Gels 7%
  15 Citations (Scopus)

  34 Downloads (Pure)

• An educational guide for nanopore sequencing in the classroom

  Salazar, A. N., Nobrega, F. L., Anyansi, C., Aparicio-Maldonado, C., Costa, A. R., Haagsma, A. C., Hiralal, A., Mahfouz, A., McKenzie, R. E., van Rossum, T., Brouns, S. J. J. & Abeel, T., 2020, In: PLoS Computational Biology. 16, 1, p. e1007314 7 p., e1007314.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Nanopore 100%
  • Nanopore Sequencing 96%
  • Bacteriophages 89%
  • nanopores 86%
  • Nanopores 83%
  9 Citations (Scopus)

  127 Downloads (Pure)

• Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions

  de Jonge, P. A., Smit Sibinga, D. J. C., Boright, O. A., Costa, A. R., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 2020, In: Journal of virology. 94, 23, 12 p., e01559.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • maleic acid 100%
  • Styrene 95%
  • styrene 74%
  • Bacteriophages 67%
  • phages 62%
  3 Citations (Scopus)

• Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

  Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., Brocken, D. J. W., Hohlbein, J. & Brouns, S. J. J., 2020, In: Molecular Cell. 77, 1, p. 39-50.e10

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Interspersed Repetitive Sequences 60%
  • Bacteria 58%
  • DNA 48%
  • RNA 30%
  18 Citations (Scopus)

• Extracting Transition Rates in Particle Tracking Using Analytical Diffusion Distribution Analysis

  Vink, J. N. A., Brouns, S. J. J. & Hohlbein, J., 2020, In: Biophysical Journal. 119, 10, p. 1970-1983 14 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Single Molecule Imaging 100%
  • Kinetics 79%
  • DNA 61%
  • DNA Polymerase I 49%
  • Biological Science Disciplines 45%
  5 Citations (Scopus)

• Inactivation of RNA and DNA viruses in water by copper and silver ions and their synergistic effect

  Soliman, M. Y. M., Medema, G., Bonilla, B. E., Brouns, S. J. J. & van Halem, D., 2020, In: Water Research X. 9, 9 p., 100077.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • RNA 100%
  • Viruses 97%
  • Silver 89%
  • Silver Ion 87%
  • DNA 85%
  8 Citations (Scopus)

  15 Downloads (Pure)

• Mining moon & mars with microbes: Biological approaches to extract iron from Lunar and Martian regolith

  Volger, R., Pettersson, G. M., Brouns, S. J. J., Rothschild, L. J., Cowley, A. & Lehner, B. A. E., 2020, In: Planetary and Space Science. 184, 9 p., 104850.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • regolith 100%
  • in situ resource utilization 98%
  • microorganisms 85%
  • natural satellites 85%
  • mars 83%
  12 Citations (Scopus)

  8 Downloads (Pure)

• Prophages are associated with extensive CRISPR-Cas auto-immunity

  Nobrega, F. L., Walinga, H., Dutilh, B. E. & Brouns, S. J. J., 2020, In: Nucleic Acids Research. 48, 21, p. 12074-12084

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Prophages 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 83%
  • Immunity 59%
  • CRISPR-Cas Systems 57%
  • Genome 16%
  13 Citations (Scopus)

  10 Downloads (Pure)

• Selective loading and processing of prespacers for precise CRISPR adaptation

  Kim, S., Loeff, L., Colombo, S., Jergic, S., Brouns, S. J. J. & Joo, C., 2020, In: Nature. 579, 7797, p. 141-145 5 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Exonucleases 58%
  • Intergenic DNA 56%
  • Single-Stranded DNA 52%
  • DNA 48%
  23 Citations (Scopus)
• 2019

  Cas4-Cas1 fusions drive efficient PAM selection and control CRISPR adaptation

  Almendros, C., Nobrega, F. L., McKenzie, R. E. & Brouns, S. J. J., 2019, In: Nucleic Acids Research. 47, 10, p. 5223-5230

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Gene Fusion 65%
  • Geobacter 50%
  • CRISPR-Cas Systems 46%
  • Immunologic Memory 40%
  21 Citations (Scopus)

  103 Downloads (Pure)

• Coacervate-based detection of a compound of interest

  Brouns, S., Reese, L. & Spoelstra, K., 2019, IPC No. C12Q, Priority date 20 Oct 2017, Priority No. NL2019775

  Research output: Patent

• Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems

  Kieper, S. N., Almendros, C. & Brouns, S. J. J., 2019, In: FEMS Microbiology Letters. 366, 11, 5 p., fnz129.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR-Cas Systems 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 72%
  • Synechocystis 23%
  • Integrases 10%
  • Conserved Sequence 9%
  8 Citations (Scopus)

  65 Downloads (Pure)

• Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

  Lehner, B. A. E., Janssen, V. A. E. C., Spiesz, E. M., Benz, D., Brouns, S. J. J., Meyer, A. S. & van der Zant, H. S. J., 2019, In: ChemistryOpen. 8, 7, p. 888-895

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Graphene Oxide 100%
  • Flake Like Crystal 99%
  • Graphene 88%
  • Conductance 60%
  • Reduction 57%
  11 Citations (Scopus)

  75 Downloads (Pure)

• CRISPR-Cas Systems Reduced to a Minimum

  Almendros, C., Kieper, S. N. & Brouns, S. J. J., 21 Feb 2019, In: Molecular Cell. 73, 4, p. 641-642

  Research output: Contribution to journal › Review article › Scientific › peer-review

  • CRISPR-Cas Systems 100%
  • Integrases 82%
  • Gene Editing 77%

• End-to-end mission design for microbial ISRU activities as preparation for a moon village

  Lehner, B. A. E., Schlechten, J., Filosa, A., Canals Pou, A., Mazzotta, D. G., Spina, F., Tjon, S. Y., Meyer, A. S., Brouns, S. J. J. & More Authors, 2019, In: Acta Astronautica. 162, p. 216-226

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Moon 100%
  • Iron 77%
  • Radiation shielding 43%
  • Bioreactors 34%
  • Solar system 33%
  7 Citations (Scopus)

  27 Downloads (Pure)

• Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

  Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Shah, S. A. & More Authors, 2019, In: Nature Reviews Microbiology. 18, 2, p. 67-83 17 p.

  Research output: Contribution to journal › Review article › Scientific › peer-review

  • Interspersed Repetitive Sequences 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 82%
  • Adaptive Immunity 72%
  • Publications 59%
  • Genes 57%
  525 Citations (Scopus)

• Evolution of BACON Domain Tandem Repeats in crAssphage and Novel Gut Bacteriophage Lineages

  Jonge, P. A. D., Meijenfeldt, F. A. B. V., Rooijen, L. E. V., Brouns, S. J. J. & Dutilh, B. E., 2019, In: Viruses. 11, 12, 17 p., v11121085.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Tandem Repeat Sequences 100%
  • Bacteroides 99%
  • Bacteriophages 77%
  • Carbohydrates 68%
  • Tail 21%
  9 Citations (Scopus)

  39 Downloads (Pure)

• Global phylogeography and ancient evolution of the widespread human gut virus crAssphage

  Edwards, R. A., Vega, A. A., Dutilh, B. E., Norman, H. M., Ohaeri, M. C., Levi, K., Brouns, S., de Jonge, P., Luzia De Nóbrega, F. & More Authors, 2019, In: Nature Reviews Microbiology. 4, 10, p. 1727-1736

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Phylogeography 100%
  • Viruses 39%
  • Microbiota 23%
  • Primates 21%
  • Bacteroidetes 16%

• Harnessing type I CRISPR–Cas systems for genome engineering in human cells

  Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Kennedy, R., Brouns, S. J. J. & More Authors, 2019, In: Nature Biotechnology. 37, 12, p. 1471-1477 7 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • RNA 100%
  • Ergonomics 98%
  • Genomics 86%
  • Cells 80%
  • CRISPR-Associated Proteins 74%
  41 Citations (Scopus)

• Molecular and Evolutionary Determinants of Bacteriophage Host Range

  de Jonge, P. A., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 1 Jan 2019, In: Trends in Microbiology. 27, 1, p. 51-63 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Host Specificity 100%
  • Bacteriophages 80%
  • Metagenomics 12%
  • Ecology 12%
  110 Citations (Scopus)

• Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation

  Musharova, O., Sitnik, V., Vlot, M., Savitskaya, E., Datsenko, K. A., Krivoy, A., Fedorov, I., Semenova, E., Brouns, S. J. J. & Severinov, K., 2019, In: Molecular Microbiology. 111, 6, p. 1558-1570

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR-Cas Systems 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 96%
  • Escherichia coli 58%
  • RNA 14%
  14 Citations (Scopus)

  72 Downloads (Pure)

• Using CAPTURE to detect spacer acquisition in native CRISPR arrays

  McKenzie, R. E., Almendros, C., Vink, J. N. A. & Brouns, S. J. J., 2019, In: Nature Protocols. 14, 3, p. 976-990

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Electrophoresis 83%
  • Polymerase Chain Reaction 51%
  • Immunologic Memory 30%
  • Ecosystem 25%
  8 Citations (Scopus)

• Visualisation of dCas9 target search in vivo using an open-microscopy framework

  Martens, K., van Beljouw, S. P. B., van der Els, S., Vink, J. N. A., Baas, S., Vogelaar, G. A., Brouns, S. J. J., van Baarlen, P., Kleerebezem, M. & Hohlbein, J., 2019, In: Nature Communications. 10, 1, 11 p., 3552.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Cellular Concentration 100%
  • Clustered Regularly Interspaced Short Palindromic Repeats 73%
  • Microscopy 53%
  • Plasmids 51%
  • plasmids 45%
  35 Citations (Scopus)

  76 Downloads (Pure)
• 2018

  Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes

  Vlot, M., Houkes, J., Lochs, S. J. A., Swarts, D. C., Zheng, P., Kunne, T., Mohanraju, P., Anders, C., Jinek, M., Van Der Oost, J., Dickman, M. J. & Brouns, S. J. J., 2018, In: Nucleic Acids Research. 46, 2, p. 873-885 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Bacteriophages 100%
  • DNA 56%
  • glucosylated DNA 54%
  • Interspersed Repetitive Sequences 35%
  • Bacteriophage T4 34%
  30 Citations (Scopus)

  54 Downloads (Pure)

• Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

  Kieper, S. N., Almendros, C., Behler, J., McKenzie, R. E., Luzia De Nóbrega, F., Haagsma, A. C., Vink, J. N. A., Hess, W. R. & Brouns, S. J. J., 2018, In: Cell Reports. 22, 13, p. 3377-3384 8 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • DNA Sequence 45%
  • Protein 35%
  • Synechocystis 24%
  • Interspersed Repetitive Sequences 11%
  66 Citations (Scopus)

  34 Downloads (Pure)

• Complete genome sequence of the Escherichia coli phage Ayreon

  Vlot, M., Nobrega, F. L., Wong, C. F. A., Liu, Y. & Brouns, S. J. J., 2018, In: Genome Announcements. 6, 2, e01354-17.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Coliphages 100%
  • Genome 44%
  1 Citation (Scopus)

  25 Downloads (Pure)

• Complete genome sequences of two T4-like Escherichia coli bacteriophages

  Costa, A. R., Brouns, S. J. J. & Nobrega, F. L., 1 Jun 2018, In: Genome Announcements. 6, 26, e00586-18.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Bacteriophages 100%
  • Escherichia coli 70%
  • Genome 68%
  • Biotechnology 38%
  • Proteins 12%
  1 Citation (Scopus)

  33 Downloads (Pure)

• Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps

  Loeff, L., Brouns, S. J. J. & Joo, C., 2018, In: Molecular Cell. 70, 3, p. 385-394.e3

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Nucleotides 100%
  • DNA 69%
  • Clustered Regularly Interspaced Short Palindromic Repeats 60%
  • E coli ygcB protein 45%
  • RNA 24%
  32 Citations (Scopus)

• Role of nucleotide identity in effective CRISPR target escape mutations

  Künne, T., Zhu, Y., da Silva, F., Konstantinides, N., McKenzie, R. E., Jackson, R. N. & Brouns, S. J., 2018, In: Nucleic Acids Research. 46, 19, p. 10395-10404

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • Guanine 77%
  • Nucleotides 70%
  • Nucleic Acid Conformation 64%
  • Cytosine 51%
  5 Citations (Scopus)

• Targeting mechanisms of tailed bacteriophages

  Nobrega, F. L., Vlot, M., de Jonge, P. A., Dreesens, L. L., Beaumont, H. J. E., Lavigne, R., Dutilh, B. E. & Brouns, S. J. J., 2018, In: Nature Reviews Microbiology. 14 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Bacteriophages 100%
  • Host Specificity 31%
  • Ecology 30%
  • Biotechnology 29%
  • Cell Surface Receptors 27%
  160 Citations (Scopus)
• 2017

  CRISPR-Cas: Adapting to change

  Jackson, S. A., McKenzie, R. E., Fagerlund, R. D., Kieper, S. N., Fineran, P. C. & Brouns, S. J. J., 7 Apr 2017, In: Science. 356, 6333, p. 1-9 9 p., eaal5056.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR-Cas systems 100%
  • adaptive immunity 83%
  • interspersed repetitive sequences 45%
  • Archaea 36%
  • neutralization 36%
  199 Citations (Scopus)

  117 Downloads (Pure)
• 2016

  Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

  Künne, T., Kieper, S. N., Bannenberg, J. W., Vogel, A. I. M., Miellet, W. R., Klein, M., Depken, M., Suarez-Diez, M. & Brouns, S. J. J., 1 Sep 2016, In: Molecular Cell. 63, 5, p. 852-864 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • DNA 48%
  • Immunologic Memory 30%
  • Thymine 29%
  • Cytosine 25%
  81 Citations (Scopus)

  177 Downloads (Pure)
• 2015

  Two distinct DNA binding modes guide dual roles of a CRISPR-cas protein complex

  Blosser, T. R., Loeff, L., Westra, E. R., Vlot, M., Künne, T., Sobota, M., Dekker, C., Brouns, S. J. J. & Joo, C., 2015, In: Molecular Cell. 58, 1, p. 60-70

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats 100%
  • DNA 48%
  • Immunity 47%
  • Proteins 32%
  • Seeds 29%
  73 Citations (Scopus)