S.J.J. Brouns

Research Output

Research Output per year

2015 2020

• 28 Article
• 2 Review article
• 1 Letter
• 1 Patent

Research Output per year

Research Output per year

Search results

• 2020

  Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to Hosts

  de Jonge, P. A., von Meijenfeldt, F. A. B., Costa, A. R., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 2020, In : iScience. 23, 9, 101439.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Adsorption
  • Bacteriophages
  • Waste Water
  • Electrophoresis
  • Gels
  1 Citation (Scopus)

  8 Downloads (Pure)

• An educational guide for nanopore sequencing in the classroom

  Salazar, A. N., Nobrega, F. L., Anyansi, C., Aparicio-Maldonado, C., Costa, A. R., Haagsma, A. C., Hiralal, A., Mahfouz, A., McKenzie, R. E., van Rossum, T., Brouns, S. J. J. & Abeel, T., 2020, In : PLoS Computational Biology. 16, 1, p. e1007314 7 p., e1007314.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Nanopore
  • nanopores
  • Nanopores
  • Bacteriophages
  • Sequencing
  4 Citations (Scopus)

  98 Downloads (Pure)

• Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions

  de Jonge, P. A., Smit Sibinga, D. J. C., Boright, O. A., Costa, A. R., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 2020, In : Journal of virology. 94, 23, 12 p., e01559.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • maleic acid
  • Styrene
  • styrene
  • Bacteriophages
  • bacteriophages

• Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

  Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., Brocken, D. J. W., Hohlbein, J. & Brouns, S. J. J., 2020, In : Molecular Cell. 77, 1, p. 39-50.e10

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Interspersed Repetitive Sequences
  • Bacteria
  • DNA
  • RNA
  8 Citations (Scopus)

• Extracting Transition Rates in Particle Tracking Using Analytical Diffusion Distribution Analysis

  Vink, J. N. A., Brouns, S. J. J. & Hohlbein, J., 2020, In : Biophysical Journal. 119, 10, p. 1970-1983 14 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Kinetics
  • DNA
  • DNA Polymerase I
  • Biological Science Disciplines
  • Clustered Regularly Interspaced Short Palindromic Repeats

• Mining moon & mars with microbes: Biological approaches to extract iron from Lunar and Martian regolith

  Volger, R., Pettersson, G. M., Brouns, S. J. J., Rothschild, L. J., Cowley, A. & Lehner, B. A. E., 2020, In : Planetary and Space Science. 184, 9 p., 104850.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • regolith
  • in situ resource utilization
  • microorganisms
  • natural satellites
  • mars

• Prophages are associated with extensive CRISPR-Cas auto-immunity

  Nobrega, F. L., Walinga, H., Dutilh, B. E. & Brouns, S. J. J., 2020, In : Nucleic Acids Research. 48, 21, p. 12074-12084

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Prophages
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Immunity
  • CRISPR-Cas Systems
  • Genome
  1 Downloads (Pure)

• Selective loading and processing of prespacers for precise CRISPR adaptation

  Kim, S., Loeff, L., Colombo, S., Jergic, S., Brouns, S. J. J. & Joo, C., 2020, In : Nature. 579, 7797, p. 141-145 5 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Exonucleases
  • Intergenic DNA
  • Single-Stranded DNA
  • DNA
  7 Citations (Scopus)
• 2019

  Cas4-Cas1 fusions drive efficient PAM selection and control CRISPR adaptation

  Almendros, C., Nobrega, F. L., McKenzie, R. E. & Brouns, S. J. J., 2019, In : Nucleic Acids Research. 47, 10, p. 5223-5230

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Gene Fusion
  • Geobacter
  • CRISPR-Cas Systems
  • Immunologic Memory
  9 Citations (Scopus)

  60 Downloads (Pure)

• Coacervate-based detection of a compound of interest

  Brouns, S., Reese, L. & Spoelstra, K., 2019, IPC No. C12Q, Priority date 20 Oct 2017, Priority No. NL2019775

  Research output: Patent

• Conserved motifs in the CRISPR leader sequence control spacer acquisition levels in Type I-D CRISPR-Cas systems

  Kieper, S. N., Almendros, C. & Brouns, S. J. J., 2019, In : FEMS Microbiology Letters. 366, 11, 5 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR-Cas Systems
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Synechocystis
  • Integrases
  • Conserved Sequence
  4 Citations (Scopus)

  54 Downloads (Pure)

• Creation of Conductive Graphene Materials by Bacterial Reduction Using Shewanella Oneidensis

  Lehner, B. A. E., Janssen, V. A. E. C., Spiesz, E. M., Benz, D., Brouns, S. J. J., Meyer, A. S. & van der Zant, H. S. J., 2019, In : ChemistryOpen. 8, 7, p. 888-895

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Graphene oxide
  • Graphite
  • Electron beam lithography
  • Nanostructured materials
  • Biosensors
  5 Citations (Scopus)

  54 Downloads (Pure)

• CRISPR-Cas Systems Reduced to a Minimum

  Almendros, C., Kieper, S. N. & Brouns, S. J. J., 21 Feb 2019, In : Molecular Cell. 73, 4, p. 641-642

  Research output: Contribution to journal › Review article › Scientific › peer-review

  • CRISPR-Cas Systems
  • Integrases
  • Gene Editing

• End-to-end mission design for microbial ISRU activities as preparation for a moon village

  Lehner, B. A. E., Schlechten, J., Filosa, A., Canals Pou, A., Mazzotta, D. G., Spina, F., Tjon, S. Y., Meyer, A. S., Brouns, S. J. J. & More Authors, 2019, In : Acta Astronautica. 162, p. 216-226

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Moon
  • Iron
  • Radiation shielding
  • Bioreactors
  • Solar system
  2 Citations (Scopus)

• Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

  Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Shah, S. A. & More Authors, 2019, In : Nature Reviews Microbiology. 17 p.

  Research output: Contribution to journal › Review article › Scientific › peer-review

  • Interspersed Repetitive Sequences
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Adaptive Immunity
  • Publications
  • Signal Transduction
  104 Citations (Scopus)

• Evolution of BACON Domain Tandem Repeats in crAssphage and Novel Gut Bacteriophage Lineages

  Jonge, P. A. D., Meijenfeldt, F. A. B. V., Rooijen, L. E. V., Brouns, S. J. J. & Dutilh, B. E., 2019, In : Viruses. 11, 12, 17 p., v11121085.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Bacteroides
  • Tandem Repeat Sequences
  • Bacteriophages
  • Carbohydrates
  • Tail
  4 Citations (Scopus)

  26 Downloads (Pure)

• Global phylogeography and ancient evolution of the widespread human gut virus crAssphage

  Edwards, R. A., Vega, A. A., Dutilh, B. E., Norman, H. M., Ohaeri, M. C., Levi, K., Brouns, S., de Jonge, P., Luzia De Nóbrega, F. & More Authors, 2019, In : Nature Microbiology. 4, 10, p. 1727-1736

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Phylogeography
  • Viruses
  • Microbiota
  • Primates
  • Bacteroidetes
  32 Citations (Scopus)

• Harnessing type I CRISPR–Cas systems for genome engineering in human cells

  Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Kennedy, R., Brouns, S. J. J. & More Authors, 2019, In : Nature Biotechnology. 37, 12, p. 1471-1477 7 p.

  Research output: Contribution to journal › Letter › Scientific › peer-review

  • RNA
  • Ergonomics
  • Cells
  • CRISPR-Associated Proteins
  • Genes
  15 Citations (Scopus)

• Molecular and Evolutionary Determinants of Bacteriophage Host Range

  de Jonge, P. A., Nobrega, F. L., Brouns, S. J. J. & Dutilh, B. E., 1 Jan 2019, In : Trends in Microbiology. 27, 1, p. 51-63 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Host Specificity
  • Bacteriophages
  • Metagenomics
  • Ecology
  43 Citations (Scopus)

• Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation

  Musharova, O., Sitnik, V., Vlot, M., Savitskaya, E., Datsenko, K. A., Krivoy, A., Fedorov, I., Semenova, E., Brouns, S. J. J. & Severinov, K., 2019, In : Molecular Microbiology. 111, 6, p. 1558-1570

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • CRISPR-Cas Systems
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Escherichia coli
  • RNA
  6 Citations (Scopus)

  56 Downloads (Pure)

• Using CAPTURE to detect spacer acquisition in native CRISPR arrays

  McKenzie, R. E., Almendros, C., Vink, J. N. A. & Brouns, S. J. J., 2019, In : Nature Protocols. 14, 3, p. 976-990

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Electrophoresis
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Polymerase Chain Reaction
  • Bacteriophages
  • Immunologic Memory
  4 Citations (Scopus)

• Visualisation of dCas9 target search in vivo using an open-microscopy framework

  Martens, K., van Beljouw, S. P. B., van der Els, S., Vink, J. N. A., Baas, S., Vogelaar, G. A., Brouns, S. J. J., van Baarlen, P., Kleerebezem, M. & Hohlbein, J., 2019, In : Nature Communications. 10, 1, 11 p., 3552.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Microscopic examination
  • Screening
  • Microscopy
  • Plasmids
  13 Citations (Scopus)

  51 Downloads (Pure)
• 2018

  Bacteriophage DNA glucosylation impairs target DNA binding by type I and II but not by type V CRISPR–Cas effector complexes

  Vlot, M., Houkes, J., Lochs, S. J. A., Swarts, D. C., Zheng, P., Kunne, T., Mohanraju, P., Anders, C., Jinek, M., Van Der Oost, J., Dickman, M. J. & Brouns, S. J. J., 2018, In : Nucleic Acids Research. 46, 2, p. 873-885 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Bacteriophages
  • DNA
  • glucosylated DNA
  • Interspersed Repetitive Sequences
  • Bacteriophage T4
  19 Citations (Scopus)

  37 Downloads (Pure)

• Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

  Kieper, S. N., Almendros, C., Behler, J., McKenzie, R. E., Luzia De Nóbrega, F., Haagsma, A. C., Vink, J. N. A., Hess, W. R. & Brouns, S. J. J., 2018, In : Cell Reports. 22, 13, p. 3377-3384 8 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Synechocystis
  • Interspersed Repetitive Sequences
  • Immunologic Memory
  • Intergenic DNA
  41 Citations (Scopus)

  19 Downloads (Pure)

• Complete genome sequence of the Escherichia coli phage Ayreon

  Vlot, M., Nobrega, F. L., Wong, C. F. A., Liu, Y. & Brouns, S. J. J., 2018, In : Genome Announcements. 6, 2, e01354-17.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Coliphages
  • Genome
  1 Citation (Scopus)

  21 Downloads (Pure)

• Complete genome sequences of two T4-like Escherichia coli bacteriophages

  Costa, A. R., Brouns, S. J. J. & Nobrega, F. L., 1 Jun 2018, In : Genome Announcements. 6, 26, e00586-18.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Bacteriophages
  • Escherichia coli
  • Genome
  • Biotechnology
  • Proteins
  1 Citation (Scopus)

  26 Downloads (Pure)

• Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps

  Loeff, L., Brouns, S. J. J. & Joo, C., 2018, In : Molecular Cell. 70, 3, p. 385-394.e3

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Nucleotides
  • DNA
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • E coli ygcB protein
  • RNA
  21 Citations (Scopus)

• Role of nucleotide identity in effective CRISPR target escape mutations

  Künne, T., Zhu, Y., da Silva, F., Konstantinides, N., McKenzie, R. E., Jackson, R. N. & Brouns, S. J., 2018, In : Nucleic Acids Research. 46, 19, p. 10395-10404

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Guanine
  • Nucleotides
  • Nucleic Acid Conformation
  • Cytosine
  3 Citations (Scopus)

• Targeting mechanisms of tailed bacteriophages

  Nobrega, F. L., Vlot, M., de Jonge, P. A., Dreesens, L. L., Beaumont, H. J. E., Lavigne, R., Dutilh, B. E. & Brouns, S. J. J., 2018, In : Nature Reviews Microbiology. 14 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Bacteriophages
  • Host Specificity
  • Ecology
  • Biotechnology
  • Cell Surface Receptors
  69 Citations (Scopus)
• 2017

  CRISPR-Cas: Adapting to change

  Jackson, S. A., McKenzie, R. E., Fagerlund, R. D., Kieper, S. N., Fineran, P. C. & Brouns, S. J. J., 7 Apr 2017, In : Science. 356, 6333, p. 1-9 9 p., eaal5056.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • adaptive immunity
  • interspersed repetitive sequences
  • Archaea
  • neutralization
  • RNA
  143 Citations (Scopus)

  94 Downloads (Pure)
• 2016

  Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

  Künne, T., Kieper, S. N., Bannenberg, J. W., Vogel, A. I. M., Miellet, W. R., Klein, M., Depken, M., Suarez-Diez, M. & Brouns, S. J. J., 1 Sep 2016, In : Molecular Cell. 63, 5, p. 852-864 13 p.

  Research output: Contribution to journal › Article › Scientific › peer-review

  Open Access

  File

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA
  • Immunologic Memory
  • Thymine
  • Cytosine
  64 Citations (Scopus)

  154 Downloads (Pure)
• 2015

  Two distinct DNA binding modes guide dual roles of a CRISPR-cas protein complex

  Blosser, T. R., Loeff, L., Westra, E. R., Vlot, M., Künne, T., Sobota, M., Dekker, C., Brouns, S. J. J. & Joo, C., 2015, In : Molecular Cell. 58, 1, p. 60-70

  Research output: Contribution to journal › Article › Scientific › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA
  • Immunity
  • Proteins
  • Seeds
  69 Citations (Scopus)